Faculty of Science
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Item Advances in composite integer factorization(International Knowledge Sharing Platiform, 2013-01-01) Wanambisi, Adrin W.; Aywa, Shem; Maende, Cleophas; Muketha, Geoffrey MuchiriIn this research we propose a new method of integer factorization. Prime numbers are the building blocks of arithmetic. At the moment there are no efficient methods (algorithms) known that will determine whether a given integer is prime or and its prime factors[1]. This fact is the basis behind many of the cryptosystems currently in use.Item Aerosol optical depth and precipitation rate projections over east africa utilizing self organizing map(The International Journal Of Science & Technoledge, 2017-03-01) Makokha, John Wanjala; Angeyo, H.K.; Muthama, John NziokaAssessment of future aerosols impacts on both regional and global climate change requires a comprehensive projection tool that reliably provides information on aerosol evolution characteristics with high fidelity. In the current study, we propose an algorithm based on Self-Organizing Map (SOM) and Community Atmosphere Model 4 (CAM4) for long term Aerosol Optical Depth (AOD) and Precipitation Rate (PR) projections over East Africa. To start with, AOD and PR retrievals from Moderate Resolution Imaging Spectroradiometer (MODIS) and Tropical Rainfall Measurement Mission (TRMM) respectively were cross validated with simulation from CAM4 so as to assess the uncertainty between the measured and simulated retrievals from 2000 to 2014.The error analysis between CAM4 simulations and MODIS measurements (from 2000 to 2014)shows a close match where R2 varies from 0.58 to 0.83 with a corresponding RMSE of between 0.014 and 0.065 (for AOD). Likewise, the uncertainty between simulate and measured PR from CAM4 and TRMM showed an estimated R2 to range between 0.40 and 0.78 while the RMSE varied from 0.021 to 0.091 in the same period and study sites. Based on proposed SOM algorithm and simulated CAM4 retrievals over each study site, an increase of between 1.34-2.43 % for AOD and a decrease of between 1.03-1.98 % in PR are projected over the region by 2030.Item Algebraic approach to composite integer factorization(European Centre for Research, Training and Development : IJMSS, 2013-03-01) Wanambisi, Adrin W.; Aywa, Shem; Maende, cleophas; Muketha, Geoffrey MuchiriThere various algorithms that can factor large integers but very few of these algorithms run in polynomial time. This fact makes them inefficient. The apparent difficulty of factoring large integers is the basis of some modern cryptographic algorithms. In this paper we propose an algebraic approach to factoring composite integer. This approach reduces the number of steps to a finite number of possible differences between two primes.Item Antimicrobial Coumarins from the Oyster Culinary-Medicinal Mushroom, Pleurotus ostreatus (Agaricomycetes), from Kenya(International Journal of Medicinal Mushrooms, 2016-01-01) Baraza, Lilechi D.; Wekesa, Wekesa; Korir, Cheruiyot Jackson; Juma, Fredrick B.; Ochieno, Dennis; Wairimu, Kamau R.; Osogo, Aggrey; Heydenreich, MatthiasPleurotus ostreatus has been widely used as food because of its nutritional and medicinal properties. These have been attributed to the presence of macronutrients, minerals, vitamins, and amino acids, among other secondary metabolites. There are, however, few reports on the antimicrobial activities of different classes of purified compounds from P. ostreatus. This led to the current study, the objective of which was to chemically characterize the antibiotic activities of P. ostreatus against selected human pathogenic bacteria and endophytic fungi. Chemical structures were determined using spectroscopic methods and by comparison with values of related structures reported in the literature. Pure compounds from P. ostreatus were tested in vitro against pathogenic bacteria (Staphylococcus aureus and Escherichia coli) and endophytic fungi (Pencillium digitatum and Fusarium proliferatum). A new compound, (E)-5,7-dimethoxy-6-(3-methylbuta-1,3-dienyl)-2H-chromen-2-one (5-methoxy-(E)-suberodiene) (compound 2), along with ergosterol (compound 1) and 5,7-dimethoxy-6-(3-methylbut-2-enyl)-2H-chromen-2-one (toddaculin; compound 3), were isolated from the fruiting bodies of P. ostreatus. The growth of S. aureus, F. proliferatum, and P. digitatum colonies was inhibited in media containing compound 2, with minimum inhibitory concentrations closely comparable to those of conventional antibiotics.Item Autoregulation of ccl26 synthesis and secretion in a549 cells: a possible mechanism by which alveolar epithelial cells modulate airway inflammation(2005) Abonyo, B. O.; Alexander, M. S. Alexander; Heiman, A. S.Abonyo, B. O., M. S. Alexander, and A. S. Heiman. Autoregulation of CCL26 synthesis and secretion in A549 cells: a possible mechanism by which alveolar epithelial cells modulate airway inflammation. Am J Physiol Lung Cell Mol Physiol 289: L478 –L488, 2005. First published April 29, 2005; doi:10.1152/ajplung.00032.2005.— Eotaxins (CCL11, CCL24, CCL26) originating from airway epithelial cells and leukocytes have been detected in bronchoalveolar lavage of asthmatics. Although the alveolar epithelium is the destination of uncleared allergens and other inflammatory products, scanty information exists on their contribution to the generation and regulation of the eotaxins. We envisioned a state whereby alveolar type II cells, a known source of other inflammatory proteins, could be involved in both the production and regulation of CCL24 and CCL26. Herein, we demonstrated that all three eotaxins are constitutively expressed in A549 cells. IL-4 and IL-13 stimulated a concentration-dependent secretion of CCL24 and CCL26. The cytokines did not act synergistically. Cycloheximide and actinomycin D abrogated IL-4- and IL13-dependent CCL26 but not CCL24 secretion. Both IL-13 and IL-4 stimulated CCL26 synthesis that was inhibited in a concentrationdependent manner by CCL26 but not CCL24. Only CCL26 reduced expression of CCR3 receptors by 30 – 40%. On the other hand, anti-CCR3 pretreatment reduced IL-4 IL-13-dependent CCL26 secretion, implying autoregulation. A CCR3-specific antagonist (SB328437) significantly decreased IL-4-dependent synthesis and release of CCL26. Eosinophils treated with medium from IL-4-stimulated A549 cells preincubated with anti-CCL26 showed a marked decrease of superoxide anion production compared with anti-CCL24 treated. These results suggest that CCL26 is a major eotaxin synthesized and released by alveolar epithelial cells and is involved in autoregulation of CCR3 receptors and other eotaxins. This CCL26-CCR3 ligandreceptor system may be an attractive target for development of therapeutics that limits progress of inflammation in airway diseaseItem Biodegradation of carbofuran in soils within Nzoia River Basin, Kenya(Journal of Environmental Science and Health, 2015-04-06) Onunga, Daniel O.; Owino, Isaac; Ngigi, Anastacia; Osogo, Aggrey; Orata, Francis; Getenga, Zachary; were, HassanCarbofuran (2,3-dihydro-2,2-dimethylbenzofuran-7-yl methylcarbamate) has been used within the Nzoia River Basin (NRB), especially in Bunyala Rice Irrigation Schemes, in Kenya for the control of pests. In this study, the capacity of native bacteria to degrade carbofuran in soils from NRB was investigated. A gram positive, rod-shaped bacteria capable of degrading carbofuran was isolated through liquid cultures with carbofuran as the only carbon and nitrogen source. The isolate degraded 98% of 100-μg mL−1 carbofuran within 10 days with the formation of carbofuran phenol as the only detectable metabolite. The degradation of carbofuran was followed by measuring its residues in liquid cultures using high performance liquid chromatography (HPLC). Physical and morphological characteristics as well as molecular characterization confirmed the bacterial isolate to be a member of Bacillus species. The results indicate that this strain of Bacillus sp. could be considered as Bacillus cereus or Bacillus thuringiensis with a bootstrap value of 100% similar to the 16S rRNA gene sequences. The biodegradation capability of the native strains in this study indicates that they have great potential for application in bioremediation of carbofuran-contaminated soil sites.Item Building Materials from Stabilized Soils: The use of under-utilized resources for Low Cost Building(International Journal of Sustainable Construction Engineering and Technology, 2013-01-01) Kwach, Boniface OtienoItem CCL26-targeted siRNA treatment of alveolar type II cells decreases expression of CCR3-binding chemokines and reduces eosinophil migration: implications in asthma therapy(2009) Errahali, Younes J.; Taka, Equar; Abonyo, B. O.; Heiman, Ann S.The underlying infl ammation present in chronic airway diseases is orchestrated by increased expression of CC chemokines that selectively recruit leukocyte populations into the pulmonary system. Human CCL26 signals through CC chemokine receptor 3 (CCR3), is dramatically upregulated in challenged asthmatics, and stimulates recruitment of eosinophils (EOSs) and other leukocytes. CCL26 participates in regulation of its receptor CCR3 and modulates expression of a variety of chemokines in alveolar type II cells. Utilizing the A549 alveolar type II epithelial cell culture model, we carried out studies to test the hypothesis that CCL26-siRNA treatment of these cells would ameliorate Th2-driven release of the eotaxins and other CCR3 ligands that would, in turn, decrease recruitment and activation of EOSs. Results demonstrate that CCL26-siRNA treatments decreased interleukin4-induced CCL26 and CCL24 expression by > 70%. CCL26-directed small-interfering RNA (siRNA) treatments signifi cantly decreased release of CCL5 (RANTES), CCL15 (MIP-1δ), CCL8 (MCP-2), and CCL13 (MCP-4). In bioactivity assays it was shown that EOS migration and activation were reduced up to 80% and 90%, respectively, when exposed to supernatants of CCL26-siRNA-treated cells. These results provide evidence that CCL26 may be an appropriate target for development of new therapeutic agents designed to alleviate the underlying infl ammation associated with chronic diseases of the airways.Item Characterization and evaluation of repellent effect of essential oil of mangifera indica l. from Kenya(Journal of Essential Oil Bearing Plants, 2013-03-12) Alwala, J.O.; Wanzala, W.; Inyambukho, RA.Ethnopharmacologically, the mango has a lot of applications in life in humanhealth and ethnoveterinary medicines since ancient times. The study aimed at characterizing theessential oil of Mangifera indica L. leaves and evaluating its repellent effect on the host-seekingfemale Anopheles gambiae,the vector of African malaria. The essential oil was obtained byhydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-massspectrometry (GC-MS). The repellent effect of the essential oil was evaluated using the human-baittechnique to simulate field situation. Of the 26 major hydrocarbon compounds identified, α-pineneoccurred in the largest amount (33.3 %), followed by α-phellandrene (22.6 %), Limonene (13.2 %),p-cymene (6.1 %), Heptane (3.8 %), β-pinene (2.6 %), Ledene (1.3 %), (-)-α-gurjunene (1.2 %), β-myrcene (1.1 %), γ-terpinene (1.0 %), (+)-2-carene (0.9 %) and trans (β)-caryophyllene (0.9 %) inthat order. The oil showed a significant dose-dependent repellent effect on host-seeking femaleAnopheles gambiae s.s. The oil showed a complex composition of hydrocarbon compounds andmay be richer in monoterpenes than in any other type of compounds. It showed the potential to repelmosquitoes.Item Characterization of -soluble n-ethylmaleimide–sensitive fusion attachment protein in alveolar type ii cells implications in lung surfactant secretion(2002) Abonyo, B. O.; Wang, Pengcheng; Narasaraju, Telugu A.; Rowan III, William H.; Zimmerman, Un-Jin; Lin LiuN-ethylmaleimide–sensitive fusion protein (NSF) and soluble NSF attachment protein (α-SNAP) are thought to be soluble factors that transiently bind and disassemble SNAP receptor complex during exocytosis in neuronal and endocrine cells. Lung surfactant is secreted via exocytosis of lamellar bodies from alveolar epithelial type II cells. However, the secretion of lung surfactant is a relatively slow process, and involvement of SNAP receptor and its cofactors (NSF and α-SNAP) in this process has not been demonstrated. In this study, we investigated a possible role of α-SNAP in surfactant secretion. α-SNAP was predominantly associated with the membranes in alveolar type II cells as determined by Western blot and immunocytochemical analysis using confocal microscope. Membrane-associated α-SNAP was not released from the membrane fraction when the cells were lyzed in the presence of Ca2+ or Mg2+ATP. The alkaline condition (0.1 M Na2CO3, pH 12), known to extract peripheral membrane proteins also failed to release it from the membrane. Phase separation using Triton X-114 showed that α-SNAP partitioned into both aqueous and detergent phases. NSF had membrane-bound characteristics similar to α-SNAP in type II cells. Permeabilization of type II cells with β-escin resulted in a partial loss of α-SNAP from the cells, but cellular NSF was relatively unchanged. Addition of exogenous α-SNAP to the permeabilized cells increased surfactant secretion in a dose-dependent manner, whereas exogenous NSF has much less effects. An α-SNAP antisense oligonucleotide decreased its protein level and inhibited surfactant secretion. Our results suggest a role of α-SNAP in lung surfactant secretion.Item Characterization of codes of ideals of the polynomial ring for control in computer applicatons(C o u n c i l f o r I n n o v a t i v e R e s e a r c h : J o u r n a l o f A d v a n c e s i n M a t h e m a t i c s, 2016-06-01) Olege, Fanuel; Owino, M.O.; Aywa, Shem; Okaka, Colleta A.The study of ideals in algebraic number system has contributed immensely in preserving the notion of unique factorization in rings of algebraic integers and in proving Fermat’s last Theorem. Recent research has revealed that ideals in Noetherian rings are closed in polynomial addition and multiplication.This property has been used to characterize the polynomial ring F_2^n[x] mod(xn-1) for error control. In this research we generate ideals of the polynomial ring using GAP software and characterize the polycodewords using Shannon’s Code region and Manin’s bound.Item Chemical composition of essential oil oftithonia diversifolia(hemsl.) a. gray from the southern slopes of Mount Elgon in western Kenya(I J E P P, 2016-06-01) Wanzala, W.; Osundwa, E.M.; Alwala, O.J.; Gakuubi, M.M.Ethnopharmacologically, Tithonia diversifolia has a lot of applications in the history of human life. The current study aimed at characterizing the essential oil from fresh aerial parts of T. diversifolia. The plant materials were obtained from western Kenya and oil extracted by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Compounds were identified by comparison of their mass spectra with those in Wiley NBS and NIST databases and GC retention times to those of authentic samples. The percentage yield of the essential oil of T. diversifoliawas 0.00015% w/w. The oil showed a complex composition of about 50 compounds, a mixture of monoterpenes and sesquiterpenes, 54% and 46%, respectively.Within the sub-classes of terpenes, hydrogen-carbon-containing terpenes (72%) occurred more than oxygen-containing terpenes (28%) with hydrogen-carbon-containing sesquiterpenes (38%) occurring in the highest proportion, followed by hydrogen-carbon-containing monoterpenes (34%), oxygenated monoterpenes (20%) and oxygenated sesquiterpenes (8%). Twenty-four percent of the 50 compounds and most of the monoterpenes were found in literature to have some repellence properties. Of these 50 compounds, α-pinene occurred in the largest amount (63.64%), followed by β-pinene (15.0%), iso-caryophyllene (7.62%), nerolidol (3.70%), 1-tridecanol (1.75%), limonene (1.52%), sabinene (1.00%), α-copaene (0.95%), α-gurjunene (0.56%) and cyclodecene (0.54%). With multipotential applications nature of T. diversifoliaplant and its products, these compounds may in future be useful in pharmaceutical, agricultural, food and perfumery industriesItem Controllability of a quasi-linear heat equation(Asian Academic Research Associates : AARJMD, 2015-04-01) Andanje, Mulambula; Matuya, John WanyonyiIn this article we have examined the controllability of the quasi-linear heat equation. We considered distributed controls with support in a small sub-domain. We assumed that the quasi-linear terms are locally Lipschitz. We have proved that there exists a control that drives to desired state within a finite time. Our main objective was to determine the existence, uniquiness and regularity of solutions of controllability of a quasi-linear heat equationItem Cytokine-stimulated human lung alveolar epithelial cells release eotaxin-2 (CCL24) and eotaxin-3 (CCL26)(Mary Ann Liebert, Inc, 2005) Heiman, Ann S.; Abonyo, B. O.; Darling-Reed, Selina F.; Alexander, Marilyn S.Asthma is a complex inflammatory disease characterized by a prolonged underlying airway inflammation resulting from cytokine-orchestrated signaling between many types of cells, including airway epithelial cells. Trafficking, recruitment, and activation of cells in airway disease are, in part, modulated by the newly discovered CC subfamily of chemokines, eotaxin (CCL11), eotaxin-2 (CCL24) and eotaxin-3 (CCL26), which transduce signals by acting as agonists for the CCR3 receptor. The specific cytokine stimuli that modulate CCL24 and CCL26 release in airway epithelial cells remain poorly defined. Thus, human 549 alveolar type II epithelium-like cells were stimulated singly and with combinations of 1–100 ng/ml tumor necrosis-factor- (TNF- ), interleukin-1 (IL-1), and IL-4, cytokines known to be elevated in the airways of asthmatics. Release of CCL11, CCL24, and CCL26 was quantified by ELISA, and CCR3 receptors monitored by immunocytochemistry and FACS analysis. Results suggest that epithelial cells release CCL11 during the first 24 h of stimulation, in contrast to a significant increase in CCL24 and CCL26 release after 24–48 h of stimulation. Differential release of the eotaxins in response to cytokine combinations was noted. The alveolar type II epithelial cells were found to possess constitutive CCR3 receptors, which increased after proinflammatory cytokine stimulation. The airway epithelium CCR3 receptor/eotaxin ligand signal transduction system may be an important target for development of novel mechanism-based adjunctive therapies designed to interrupt the underlying chronic inflammation in allergic and inflammatory disorders.Item Derivation and solution of the heat equation in 1-D(International Journal of Engineering, Science and Mathematics, 2013-06-01) Kwach, Boniface Otieno; Ongati, Omolo; Alambo, David; Okaka, Colleta A.Heat flows in the direction of decreasing temperature, that is, from hot to cool. In this paper we derive the heat equation and consider the flow of heat along a metal rod. The rod allows us to consider the temperature, u(x,t), as one dimensional in x but changing in time, t.Item Determination of tannin concentrations in african indigenous vegetables, grains and cassava roots from Emuhaya District, western Kenya(Sciknow Publications Ltd : American Journal of Nutrition and Food Science, 2014-01-01) Wanzala, W.; Alwala, J.O.; Kiema, Francis N.The studyevaluated tanninconcentrations in four vegetables, three grains and cassava roots as the most common foodstuffs in the diet of local communities from Emuhaya district in western Kenyaand their nutritional health implication. Plant specimens were collected from local arable farms and market centres and their identities confirmed at Maseno University herbarium. Tannincontent was evaluated using the International Phamacopoeia Method.Percent compositionof tannins in the sampled specimens were: 9.49±0.6 for rattle pod (Crotalaria brevidens(Emiroo); Family, Fabaceae), 8.38±0.3 for narrow-leaf bitter-pea(Daviesialeptophylla(Omurere); Family, Fabaceae), 2.49±0.2 for amaranth (Amaranthushybridus(Tsimboka); Family, Amaranthaceae), 0.42±0.04for black nightshade(Solanumnigrum(Lisutsa); Family, Solanaceae), 9.21±0.4 for peanut/groundnut (Arachishypogaea(Tsinjugu); Family, Fabaceae), 8.24±0.4 for sesame(Sesamumindicum(Tsinuni); Family, Pedaliaceae), 8.11±0.2 for finger millet (Eleusinecoracana(Obulee); Family, Poaceae) and 6.99±0.5 for cassava (Manihotesculenta(Emioko); Family, Euphorbiaceae). Solanumnigrumhad significantly the lowest tannin composition profile followed by A. hybridusand M. esculentain that order (P < 0.05). Interestingly, these three plants are preferred in the diet to the rest of the plants. The results provide scientific rationale for dietary exploitationof these plants bythe local communities and may lay down some groundwork for exploiting partially refined products such as peanut butter and a wide range of grades of flour for human consumption and livestock feeds. However, consumption of tannins in the diet may sometimes benecessary for optimal health but caution needs to be taken for their conflicting adverse medical-based nutritional and physiological effects.Item Distribution of spectrum in a direct sum decomposition of operators into normal and completely non-normal parts(Modern Scientific press : IJMMS, 2014-01-01) Mwenda, E.; Musundi, Sammy; Nzimbi, B.M; Marani, vincent Nyongesa; Loyford, NWe discuss the distribution of spectra of a direct sum decomposition of an arbitrary operator into normal and completely non normal parts. We utilize the fact that any given operator 𝑇∈𝐵(𝐻) can be decomposed into a direct summand 𝑇=𝑇1⊕𝑇2 with 𝑇1 and 𝑇2 are the normal and completely non normal parts respectively. This canonical decomposition is preferred to other forms of decomposition such as Polar and Cartesian decompositions because these two do not transfer certain properties (for instance the spectra, numerical range, and numerical radius) from the original /decomposed operator to the constituent parts. This is presumably done since these parts are simpler to deal with.Item Dynamic risk model for Rift Valley fever outbreaks in Kenya based on climate and disease outbreak data(PAGEpress-Geospatial Health, 2015-10-18) Gikungu, David; Wakhungu, Jacob; Siamba, D.N; Neyole, EdwardRift Valley fever (RVF) is a mosquito-borne viral zoonotic disease that occurs throughout sub-Saharan Africa, Egypt and the Arabian Peninsula, with heavy impact in affected countries. Outbreaks are episodic and related to climate variability, especially rainfall and flooding. Despite great strides towards better prediction of RVF epidemics, there is still no observed climate data-based warning system with sufficient lead time for appropriate response and mitigation. We present a dynamic risk model based on historical RVF outbreaks and observed meteorological data. The model uses 30-year data on rainfall, temperature, relative humidity, normalised difference vegetation index and sea surface temperature data as predictors. Our research on RVF focused on Garissa, Murang’a and Kwale counties in Kenya using a research design based on a correlational, experimental, and evaluational approach. The weather data were obtained from the Kenya Meteorological Department while the RVF data were acquired from International Livestock Research Institute, and the Department of Veterinary Services. Performance of the model was evaluated by using the first 70% of the data for calibration and the remaining 30% for validation. The assessed components of the model accurately predicted already observed RVF events. The Brier score for each of the models (ranging from 0.007 to 0.022) indicated high skill. The coefficient of determination (R2) was higher in Garissa (0.66) than in Murang’a (0.21) and Kwale (0.16). The discrepancy was attributed to data distribution differences and varying ecosystems. The model outputs should complement existing early warning systems to detect risk factors that predispose for RVF outbreaks.Item EDXRF spectroscopic elemental analysis for efficacy of Kibabii University sewage treatment System(Open Access Library Journal, 2018-10-18) Situma, Yonah; Odhiambo, Jared Oloo; Makokha, John WanjalaElemental analysis of sewage effluents in Kibabii sewage treatment system was achieved via Energy-Dispersive X-Ray Fluorescence (EDXRF) spectroscopy with the aim of assessing the efficacy of the treatment system. Concentrations of lead (Pb), mercury (Hg), cadmium (Cd) and arsenic (As) were measured based on clay soil “standards” in concentration range for Pb, Hg and As as 12.7 ppm, 14.3 ppm and 8.83 ppm respectively for certification. Among other probable reference materials, clay soil “standards” were used for certification in this work because of similarity in matrix composition with the sediments. The concentration for Hg, Pb, and As in the sewage sediments were 10.65 ± 2.28 ppm, 8.86 ± 2.92 ppm and 3.41 ± 2.18 ppm respectively in lagoon A. In lagoon B the levels were 3.82 ± 0.56 ppm, 6.35 ± 1.50 ppm and 1.67 ± 0.53 ppm respectively. Lagoon C showed a reduction in the levels with 3.74 ± 1.39 ppm for Hg and 2.30 ± 0.27 ppm for Pb. As was not detected in lagoon C, on the other hand, Cd was not detected in all the lagoons. The efficacies in the treatment varied from 30.34% - 51.78%, 37.63% - 65.41% and 76.63% - 84.81% for lagoon A, B and C respectively. The study was successful in quantifying the heavy elements in the lagoons from which the efficacy in the treatment process was determined. The study provided awareness on the elemental concentration levels in the Kibabii University sewage treatment system, hence creating awareness on what is released into River Kibabii. With this information, the surrounding community and the University can partner in order to mitigate the effects of heavy metals in the effluents in future due to the growth of the University day by day.Item Effect of duo fermion spin on the specific heat and entropy of a mixture of helium isotopes(Journal of Multidisciplinary Engineering Science and Technology (, 2018-10-01) Lusamamba, Simon Mwatulo; Sakwa, Thomas W.; Odhiambo, Jared OlooSpin normally determines the character, rate of collision and subsequently the properties of ultracold systems. This study investigated the effect of double spin degeneracy on the thermodynamic properties of Helium-3 and Helium-4 isotopes specifically the specific heat and entropy of a grand canonical ensemble. The approach used was statistical in nature where permutation and exclusion was done on fermions and bosons. From thermodynamics, the expression of partition function which was used to derive other expressions of specific heat and entropy as temperature dependence. Specific heat and entropy were found to increase with temperature. The kink in specific heat at a temperature of 35K implied that there was a phase transition.