Department of Agriculture and Veterinary Sciences

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Browsing Department of Agriculture and Veterinary Sciences by Subject "Africa"

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    Evaluating interventions uptake in indigenous chicken production in a participatory research with smallholder farmers in Kenya
    (2015) Ndegwa, Joseph M.; Mead, Roger.; Norrish, Patricia.; Shepherd, Derek.; Kimani, Catherine.; Wachira, Anne.; Siamba, Donald N.
    Indigenous chicken production system has long been characterised by low productivity due to among other factors, poor management, inadequate and poor feeding regime, poor (or lack) of disease control measures, poor hygiene, inappropriate housing, negative attitudes, lack of technical knowledge and lack of institutional support in terms of policy and infrastructure. This research was carried out to evaluate uptake by farmers of improved management practices (interventions) and their effect on performance of indigenous chickens at farm level and consequences for farmer participation in the implementation of research activities. The research involved 200 farmers in five regions in three counties. Four villages were selected per region and10 farms in each village. Interventions housing, feed supplementation, vaccination and deworming were implemented by farmers and monitoring and evaluation carried out. Farmers used own local inputs in implementing the project interventions and recorded various project activities and outputs. The project was monitored over a span of five, 3-months long periods. 25% of farmers in the entire five regions did not have housing as a treatment in any of 5 periods. Feed supplementation had high level of use by all farmers in each period. More farmers applied deworming in later periods, 25% had vaccination in period 1, and 40% in period 5. Periods 3 – 5 generally seem to be the time most applications were done. Flock sizes rose from 10 – 20 birds per farm to 20 – 30. Farmer participatory research is a tool for technology testing and transfer and a quick and effective means of generating and disseminating information.
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    A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa
    (Elsevier, 2004-04) Dinkel, Anke; Njoroge, Ernest M.; Zimmermann, Anja; Wälza, Marcus; Zeyhleb, Eberhard; Elmahdi, Ibrahim E; Mackenstedt, Ute; Romig, Thomas
    We describe the development of a specific and sensitive PCR/semi-nested PCR system for the rapid diagnosis of Echinococcus granulosus genotype G1, E. granulosus genotype G6/7, and Echinococcus ortleppi (G5). Diagnosis of G1 and the group G5/6/7 is performed by a simple PCR, while discrimination between E. ortleppi (G5) and G6/7 involves a subsequent semi-nested PCR step. The target sequence for amplification is part of the mitochondrial 12S rRNA gene. Specificity of the PCRs was 100% when evaluated with isolates of 16 species of cestodes, including Echinococcus multilocularis, Echinococcus equinus, E. ortleppi and three strains of E. granulosus (G1, G6 and G7). Sensitivity threshold was 0.25 pg of DNA. This new approach was compared with published protocols of restriction fragment length polymorphism-PCR and sequencing of mitochondrial cytochrome c oxidase subunit 1 and NADH dehydrogenase 1 genes using Echinococcus isolates of human, sheep, goat, camel, cattle and pig origin from Kenya and Sudan. Additionally, two internal DNA probes were developed, one hybridising only with G1, the other with G5, G6 and G7 amplification products. Preliminary epidemiological results obtained with this PCR approach include the detection of a camel strain (G6) infection for the first time in a human patient from eastern Africa, and the first reports of E. ortleppi (G5) in livestock from Kenya and the Sudan.