Reorganization of cytoskeleton during surfactant secretion in lung type II cells: a role of annexin II
dc.contributor.author | Singh, Taran K. | |
dc.contributor.author | Abonyo, B. O. | |
dc.contributor.author | Narasaraju, Telugu A. | |
dc.date.accessioned | 2019-06-07T13:38:25Z | |
dc.date.available | 2019-06-07T13:38:25Z | |
dc.date.issued | 2003 | |
dc.description.abstract | The secretion of lung surfactant requires the movement of lamellar bodies to the plasma membrane through cytoskeletal barrier at the cell cortex. We hypothesized that the cortical cytoskeleton undergoes a transient disassembly/reassembly in the stimulated type II cells, therefore allowing lamellar bodies access to the plasma membrane. Stabilization of cytoskeleton with Jasplakinolinde (JAS), a cell permeable actin microfilament stabilizer, caused a dose-dependent inhibition of lung surfactant secretion stimulated by terbutaline. This inhibition was also observed in ATP-, phorbol 12-myristate 13-acetate (PMA)- or Ca2 + ionophore A23187-stimulated surfactant secretion. Stimulation of type II cells with terbutaline exhibited a transient disassembly of filamentous actin (F-actin) as determined by staining with Oregon Green 488 Phalloidin. The protein kinase A inhibitor, H89, abolished the terbutaline-induced F-actin disassembly. Western blot analysis using anti-actin and anti-annexin II antibodies showed a transient increase of G-actin and annexin II in the Triton X-100 soluble fraction of terbutaline-stimulated type II cells. Furthermore, introduction of exogenous annexin II tetramer (AIIt) into permeabilized type II cells caused a disruption in the cortical actin. Treatment of type II cells with N-ethylmaleimide (NEM) resulted in a disruption of the cortical actin. NEM also inhibited annexin II’s abilities to bundle F-actin. The results suggest that cytoskeleton undergoes reorganization in the stimulated type II cells, and annexin II tetramer plays a role in this process. D 2003 Elsevier Inc. All rights reserved. | en_US |
dc.identifier.uri | http://erepository.kibu.ac.ke/handle/123456789/1181 | |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/us/ | * |
dc.subject | Cytoskeleton | en_US |
dc.subject | Surfactant | en_US |
dc.subject | Annexin II | en_US |
dc.subject | Exocytosis | en_US |
dc.title | Reorganization of cytoskeleton during surfactant secretion in lung type II cells: a role of annexin II | en_US |
dc.type | Article | en_US |
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