Syntaxin 2 and SNAP-23 are required for regulated surfactant secretion
dc.contributor.author | Abonyo, B. O. | |
dc.contributor.author | Wang, Pengcheng | |
dc.contributor.author | Lin Liu | |
dc.date.accessioned | 2019-06-07T12:29:29Z | |
dc.date.available | 2019-06-07T12:29:29Z | |
dc.date.issued | 2014 | |
dc.description.abstract | The secretion of lung surfactant in alveolar type II cells is a complex process involving the fusion of lamellar bodies with the plasma membrane. This process is somewhat different from the exocytosis of hormones and neurotransmitters. For example, it is a relatively slower process, and lamellar bodies are very large vesicles with a diameter of approximately 1 microm. SNARE proteins are the conserved molecular machinery of exocytosis in the majority of secretory cells. However, their involvement in surfactant secretion has not been reported. Here, we showed that syntaxin 2 and SNAP-23 are expressed in alveolar type II cells. Both proteins are associated with the plasma membrane, and to some degree with lamellar bodies. An antisense oligonucleotide complementary to syntaxin 2 decreased its mRNA and protein levels. The same oligonucleotide also inhibited surfactant secretion, independent of secretagogues. A peptide derived from the N-terminus of syntaxin 2 or the C-terminus of SNAP-23 significantly inhibited Ca(2+)- and GTPgammaS-stimulated surfactant secretion from permeabilized type II cells in a dose-dependent manner. Furthermore, introduction of anti-syntaxin 2 or anti-SNAP-23 antibodies into permeabilized type II cells also inhibited surfactant release. Our results suggest that syntaxin 2 and SNAP-23 are required for regulated surfactant secretion. | en_US |
dc.identifier.uri | http://erepository.kibu.ac.ke/handle/123456789/1180 | |
dc.language.iso | en | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/us/ | * |
dc.title | Syntaxin 2 and SNAP-23 are required for regulated surfactant secretion | en_US |
dc.type | Article | en_US |
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